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Issue 4, 2016
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High-throughput measurement of drug–cyclodextrin kinetic rate constants by a small molecule microarray using surface plasmon resonance imaging

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Abstract

Applications of small molecule microarrays (SMMs) has been extensively studied but have been limited to the screening of small molecule inhibitors. Here for the first time, we conjugated SMMs with label free surface plasmon resonance imaging (SPRi) for measurement of kinetic parameters for drug–cyclodextrins interactions in high-throughput manner. A collection of insoluble drugs was immobilized onto biosensor surface using photo-cross-linked technique to form SMMs. A highly sensitive and recently reported surface chemistry based on surface initiated polymerization chemistry was used for SMMs fabrication. In total, 38 insoluble drugs were evaluated for their interaction profile and kinetic rate constants against 5 different types of cyclodextrins (CDs) including, α-CD, β-CD, γ-CD, 2-hydroxylpropyl β-CD (HP-β-CD) and sulphobutyl-ether-β-CD (SBE-β-CD). For the supramolecular drug–CD interaction kinetics, the response magnitude and detailed kinetic parameters were calculated and presented in the article. The presented method described a label free and high-throughput technique for real time measurement of kinetic constants for drug–CDs interactions which will assist the selection and use of different CDs in number of different applications.

Graphical abstract: High-throughput measurement of drug–cyclodextrin kinetic rate constants by a small molecule microarray using surface plasmon resonance imaging

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Supplementary files

Article information


Submitted
14 Oct 2015
Accepted
02 Dec 2015
First published
07 Dec 2015

RSC Adv., 2016,6, 3213-3218
Article type
Paper
Author version available

High-throughput measurement of drug–cyclodextrin kinetic rate constants by a small molecule microarray using surface plasmon resonance imaging

V. Singh, Z. Li, X. Zhou, X. Xu, J. Xu, A. Nand, H. Wen, H. Li, J. Zhu and J. Zhang, RSC Adv., 2016, 6, 3213
DOI: 10.1039/C5RA21298G

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