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Issue 44, 2016
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Protein storage with perfluorinated PEG compartments in a hydrofluorocarbon solvent†

Yuta Koda,a   Takaya Terashima,*a   Heather D. Maynardb  and  Mitsuo Sawamoto*a  
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* Corresponding authors

a Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan
E-mail: terashima@living.polym.kyoto-u.ac.jp, sawamoto@star.polym.kyoto-u.ac.jp

b Department of Chemistry and Biochemistry and California Nano Systems Institute, University of California, 607 Charles E. Young Drive East, Los Angeles, CA 90095-1569, USA

Abstract

We report a novel storage technology of proteins with surface-perfluorinated poly(ethylene glycol) compartments in 2H,3H-perfluoropentane. The compartments were obtained from self-folding and self-assembly of an amphiphilic random copolymer bearing poly(ethylene glycol) and perfluoroalkyl pendants in the hydrofluorocarbon. Lysozyme and α-chymotrypsin as model proteins were efficiently encapsulated within the PEG compartments and quantitatively recovered therefrom with water. The recovered lysozyme maintained the original higher order structure without denaturation to show enzymatic activity for the hydrolysis of Micrococcus lysodeikticus as high as its original counterpart in water. The storage technology was further effective to inhibit inactivation of α-chymotrypsin.

Graphical abstract: Protein storage with perfluorinated PEG compartments in a hydrofluorocarbon solvent

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Article information

https://doi.org/10.1039/C6PY01333C
Submitted
30 Jul 2016
Accepted
31 Aug 2016
First published
01 Sep 2016
Polym. Chem., 2016,7, 6694-6698
Article type
Communication
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Protein storage with perfluorinated PEG compartments in a hydrofluorocarbon solvent

Y. Koda, T. Terashima, H. D. Maynard and M. Sawamoto, Polym. Chem., 2016, 7, 6694
DOI: 10.1039/C6PY01333C

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