Jump to main content
Jump to site search

Issue 14, 2016
Previous Article Next Article

Cellular internalization of LiNbO3 nanocrystals for second harmonic imaging and the effects on stem cell differentiation

Author affiliations

Abstract

Second harmonic generation (SHG) nanocrystals have recently been reported to label cancer cells and other functional cell lines due to their unique double-frequency property. In this paper, we report for the first time the use of lithium niobate (LiNbO3, LN) nanocrystals as SHG labels for imaging stem cells. Rat mesenchymal stem cells (rMSCs) were labeled with LN nanocrystals in order to study the cellular internalization of the nanocrystals and the influence on stem cell differentiation. The results showed that LN nanocrystals were endocytosed by the rMSCs and the distribution of the internalized nanoparticles demonstrated a high consistency with the orientation of the actin filaments. Besides, LN-labeled rMSCs showed a concentration-dependent viability. Most importantly, rMSCs labeled with 50 μg per mL of LN nanocrystals retained their ability to differentiate into both osteogenic and adipogenic lineages. The results prove that LN nanocrystals can be used as a cytocompatible, near-infrared (NIR) light driven cell label for long-term imaging, without hindering stem cell differentiation. This work will promote the use of LN nanocrystals to broader applications like deep-tissue tracking, remote drug delivery and stem cell therapy.

Graphical abstract: Cellular internalization of LiNbO3 nanocrystals for second harmonic imaging and the effects on stem cell differentiation

Back to tab navigation

Supplementary files

Publication details

The article was received on 28 Jan 2016, accepted on 09 Mar 2016 and first published on 11 Mar 2016


Article type: Communication
DOI: 10.1039/C6NR00785F
Nanoscale, 2016,8, 7416-7422

  •   Request permissions

    Cellular internalization of LiNbO3 nanocrystals for second harmonic imaging and the effects on stem cell differentiation

    J. Li, J. Qiu, W. Guo, S. Wang, B. Ma, X. Mou, M. Tanes, H. Jiang and H. Liu, Nanoscale, 2016, 8, 7416
    DOI: 10.1039/C6NR00785F

Search articles by author

Spotlight

Advertisements