Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.

Issue 1, 2016
Previous Article Next Article

De novo design of protein mimics of B-DNA

Author affiliations


Structural mimicry of DNA is utilized in nature as a strategy to evade molecular defences mounted by host organisms. One such example is the protein Ocr – the first translation product to be expressed as the bacteriophage T7 infects E. coli. The structure of Ocr reveals an intricate and deliberate arrangement of negative charges that endows it with the ability to mimic ∼24 base pair stretches of B-DNA. This uncanny resemblance to DNA enables Ocr to compete in binding the type I restriction modification (R/M) system, and neutralizes the threat of hydrolytic cleavage of viral genomic material. Here, we report the de novo design and biophysical characterization of DNA mimicking peptides, and describe the inhibitory action of the designed helical bundles on a type I R/M enzyme, EcoR124I. This work validates the use of charge patterning as a design principle for creation of protein mimics of DNA, and serves as a starting point for development of therapeutic peptide inhibitors against human pathogens that employ molecular camouflage as part of their invasion stratagem.

Graphical abstract: De novo design of protein mimics of B-DNA

Back to tab navigation

Supplementary files

Article information

02 Aug 2015
09 Nov 2015
First published
16 Nov 2015

Mol. BioSyst., 2016,12, 169-177
Article type
Author version available

De novo design of protein mimics of B-DNA

D. Yüksel, P. R. Bianco and K. Kumar, Mol. BioSyst., 2016, 12, 169
DOI: 10.1039/C5MB00524H

Search articles by author