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Issue 20, 2016
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Polymerase chain reaction in microfluidic devices

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Abstract

The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude. Since the first application of PCR in a microfluidic device was developed in 1998, an increasing number of researchers have continued the development of microfluidic PCR systems. In this review, we introduce recent developments in microfluidic-based space and time domain devices as well as discuss various designs integrated with multiple functions for sample preparation and detection. The development of isothermal nucleic acid amplification and digital PCR microfluidic devices within the last five years is also highlighted. Furthermore, we introduce various commercial microfluidic PCR devices.

Graphical abstract: Polymerase chain reaction in microfluidic devices

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Article information


Submitted
02 Aug 2016
Accepted
06 Sep 2016
First published
06 Sep 2016

This article is Open Access

Lab Chip, 2016,16, 3866-3884
Article type
Critical Review

Polymerase chain reaction in microfluidic devices

C. D. Ahrberg, A. Manz and B. G. Chung, Lab Chip, 2016, 16, 3866
DOI: 10.1039/C6LC00984K

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    [Original citation] - Published by The Royal Society of Chemistry.

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