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Issue 15, 2016
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A lab-on-a-chip device for investigating the fusion process of olfactory ensheathing cell spheroids

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Abstract

Understanding the process of fusion of olfactory ensheathing cell spheroids will lead to improvement of cell transplantation therapies to repair spinal cord injuries. The successful fusion of transplanted spheroids will enable alternative transplantation strategies to be developed for in vivo applications. This paper describes the use of a microfluidic device to trap and fuse olfactory ensheathing cell spheroids. The velocity, the pressure distribution in the device were simulated numerically to predict the trapping location. The simulation predicted the optimum flow rates for trapping the spheroids in the later experiments. Simulated particle trajectories were verified experimentally with tracing of fluorescent micro particles. The fusion process of the spheroids was investigated over a period of 48 hours. The microfluidic platform presented here can be used for testing potential drugs that can promote the fusion process and improve the transplantation therapy.

Graphical abstract: A lab-on-a-chip device for investigating the fusion process of olfactory ensheathing cell spheroids

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Supplementary files

Article information


Submitted
26 Jun 2016
Accepted
04 Jul 2016
First published
04 Jul 2016

Lab Chip, 2016,16, 2946-2954
Article type
Paper

A lab-on-a-chip device for investigating the fusion process of olfactory ensheathing cell spheroids

A. Munaz, R. K. Vadivelu, J. A. S. John and N. Nguyen, Lab Chip, 2016, 16, 2946
DOI: 10.1039/C6LC00815A

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