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Issue 19, 2016

Versatile, cell and chip friendly method to gel alginate in microfluidic devices

Author affiliations

Abstract

Alginate is used extensively in microfluidic devices to produce discrete beads or fibres at the microscale. Such structures may be used to encapsulate sensitive cargoes such as cells and biomolecules. On chip gelation of alginate represents a significant challenge since gelling kinetics or physicochemical conditions are not biocompatible. Here we present a new method that offers a hitherto unprecedented level of control over the gelling kinetics and pH applied to the encapsulation of a variety of cells in both bead and fibre geometries. This versatile approach proved straightforward to adjust to achieve appropriate solution conditions required for implementation in microfluidic devices and resulted in highly reliable device operation and very high viability of several different encapsulated cell types for prolonged periods. We believe this method offers a paradigm shift in alginate gelling technology for application in microfluidics.

Graphical abstract: Versatile, cell and chip friendly method to gel alginate in microfluidic devices

Supplementary files

Article information


Submitted
16 Jun 2016
Accepted
10 Aug 2016
First published
11 Aug 2016

Lab Chip, 2016,16, 3718-3727
Article type
Paper

Versatile, cell and chip friendly method to gel alginate in microfluidic devices

A. G. Håti, D. C. Bassett, J. M. Ribe, P. Sikorski, D. A. Weitz and B. T. Stokke, Lab Chip, 2016, 16, 3718 DOI: 10.1039/C6LC00769D

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