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Issue 10, 2016

On-chip analysis, indexing and screening for chemical producing bacteria in a microfluidic static droplet array

Author affiliations

Abstract

Economic production of chemicals from microbes necessitates development of high-producing strains and an efficient screening technology is crucial to maximize the effect of the most popular strain improvement method, the combinatorial approach. However, high-throughput screening has been limited for assessment of diverse intracellular metabolites at the single-cell level. Herein, we established a screening platform that couples a microfluidic static droplet array (SDA) and an artificial riboswitch to analyse intracellular metabolite concentration from single microbial cells. Using this system, we entrapped single Escherichia coli cells in SDA to measure intracellular L-tryptophan concentrations. It was validated that intracellular L-tryptophan concentration can be evaluated by the fluorescence from the riboswitch. Moreover, high-producing strains were successfully screened from a mutagenized library, exhibiting up to 145% productivity compared to its parental strain. This platform will be widely applicable to strain improvement for diverse metabolites by developing new artificial riboswitches.

Graphical abstract: On-chip analysis, indexing and screening for chemical producing bacteria in a microfluidic static droplet array

Supplementary files

Article information


Submitted
26 Jan 2016
Accepted
08 Apr 2016
First published
08 Apr 2016

This article is Open Access

Lab Chip, 2016,16, 1909-1916
Article type
Paper

On-chip analysis, indexing and screening for chemical producing bacteria in a microfluidic static droplet array

S. Jang, B. Lee, H. Jeong, S. H. Jin, S. Jang, S. G. Kim, G. Y. Jung and C. Lee, Lab Chip, 2016, 16, 1909 DOI: 10.1039/C6LC00118A

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