Jump to main content
Jump to site search

Issue 2, 2016
Previous Article Next Article

A mesofluidic platform integrating restricted access-like sorptive microextraction as a front end to ICP-AES for the determination of trace level concentrations of lead and cadmium as contaminants in honey

Author affiliations

Abstract

An automatic programmable-flow system capitalizing upon mesofluidic Lab-On-Valve (LOV) sample processing coupled on-line to inductively coupled plasma atomic emission spectrometry (ICP-AES) is proposed in this work as a quality control tool for expedient assessment of potential contamination episodes of food safety elements (namely, Cd and Pb) in a variety of undigested ripened honeys, taken as a model of an edible matrix. On-chip micro-solid phase extraction (μSPE) is effected in a bead-injection disposable sorbent mode using a cationic exchange restricted access-like material (viz., Bond Elut Plexa PCX). We have proven that the lifetime of the miniaturized solid reactor on-chip (11 ± 0.6 mg) is limited to processing less than 6 mL of unfiltered sample suspension (5% (w/w) honey buffered at pH 4.5) after which the analytical performance is severely deteriorated because of strong adherence of the sample matrix to the bead surface. Several physicochemical parameters including the nature of the sorbent material and its ionic form were investigated in detail so as to maximize absolute recoveries and enrichment factors of Pb and Cd. Variables for the elution process were explored by means of a full factorial design. Using 4.0 mL of sample and 200 μL of 3.0 mol L−1 HNO3 as an eluent, enrichment factors of ca. 15 with extraction/elution efficiencies close to 80% and relative recoveries ranging from 90–111% in honey were obtained for both Pb and Cd. The limits of detection (LODs), based on the 3sintercept criterion, were 26 ng g−1 and 68 ng g−1 for Cd and Pb, respectively, which are far below those endorsed by current regulatory agencies (namely, 100 and 300–500 ng g−1 for Cd and Pb, respectively). Demonstrated by the analysis of a suite of off-the-shelf honey brands, the proposed LOV-μSPE platform hyphenated to ICP-AES is deemed suitable for the reliable quantitation of trace level concentrations of Pb and Cd in honey and the detection of heavy metal contamination episodes.

Graphical abstract: A mesofluidic platform integrating restricted access-like sorptive microextraction as a front end to ICP-AES for the determination of trace level concentrations of lead and cadmium as contaminants in honey

Back to tab navigation

Supplementary files

Publication details

The article was received on 14 Sep 2015, accepted on 04 Nov 2015 and first published on 04 Nov 2015


Article type: Paper
DOI: 10.1039/C5JA00387C
J. Anal. At. Spectrom., 2016,31, 473-481
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    A mesofluidic platform integrating restricted access-like sorptive microextraction as a front end to ICP-AES for the determination of trace level concentrations of lead and cadmium as contaminants in honey

    A. Sixto, M. Fiedoruk-Pogrebniak, M. Rosende, D. Cocovi-Solberg, M. Knochen and M. Miró, J. Anal. At. Spectrom., 2016, 31, 473
    DOI: 10.1039/C5JA00387C

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements