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Issue 4, 2016
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The appeasement of Doug: a synthetic approach to enhancer biology

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Genetic approaches have been instrumental in dissecting developmental enhancers by characterizing their transcription factor binding sites. Though some enhancers have been well-studied in this regard, we cannot currently build developmental enhancers from scratch. Reconstitution experiments can provide important complementary tests of our understanding of enhancer function, but these experiments are exceedingly rare in the literature, possibly due to the difficulty of publishing negative results. In this perspective, we argue that the time is right for a synthetic approach to enhancer biology. Focusing primarily on Drosophila enhancers as examples, we review classic and modern methods for dissecting enhancer function as well as computational tools for enhancer design. We include our own negative results from attempts to reconstitute the stripe 2 enhancer from the even-skipped locus and discuss possible ways forward. We believe that with a communal effort in open data sharing, we can make substantial progress toward a complete understanding of enhancer function.

Graphical abstract: The appeasement of Doug: a synthetic approach to enhancer biology

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Publication details

The article was received on 18 Dec 2015, accepted on 23 Feb 2016 and first published on 25 Feb 2016

Article type: Review Article
DOI: 10.1039/C5IB00321K
Citation: Integr. Biol., 2016,8, 475-484
  • Open access: Creative Commons BY license

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