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Issue 7, 2016
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Attenuated total reflection Fourier-transform infrared (ATR-FTIR) imaging of tissues and live cells

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Abstract

FTIR spectroscopic imaging is a label-free, non-destructive and chemically specific technique that can be utilised to study a wide range of biomedical applications such as imaging of biopsy tissues, fixed cells and live cells, including cancer cells. In particular, the use of FTIR imaging in attenuated total reflection (ATR) mode has attracted much attention because of the small, but well controlled, depth of penetration and corresponding path length of infrared light into the sample. This has enabled the study of samples containing large amounts of water, as well as achieving an increased spatial resolution provided by the high refractive index of the micro-ATR element. This review is focused on discussing the recent developments in FTIR spectroscopic imaging, particularly in ATR sampling mode, and its applications in the biomedical science field as well as discussing the future opportunities possible as the imaging technology continues to advance.

Graphical abstract: Attenuated total reflection Fourier-transform infrared (ATR-FTIR) imaging of tissues and live cells

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Publication details

The article was received on 30 Jun 2015 and first published on 21 Oct 2015


Article type: Review Article
DOI: 10.1039/C5CS00515A
Chem. Soc. Rev., 2016,45, 1850-1864

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    Attenuated total reflection Fourier-transform infrared (ATR-FTIR) imaging of tissues and live cells

    K. L. Andrew Chan and S. G. Kazarian, Chem. Soc. Rev., 2016, 45, 1850
    DOI: 10.1039/C5CS00515A

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