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Issue 32, 2016
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The effect of imidazolium cations on the structure and activity of the Candida antarctica Lipase B enzyme in ionic liquids

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Abstract

In order to understand how cations affect the structural changes and enzyme activity of Lipase B from Candida antarctica, we performed all-atom molecular dynamics simulations of CALB in four types of ionic liquids (ILs) with varying sizes of imidazolium cations and correlated these results with the experimentally determined CALB activity. The imidazolium cations under study differ in the alkyl tail length in the following order: [Emim]+ < [Bmim]+ < [Hmim]+ < [Omim]+. We observed that the best enzyme activity and structural stability of CALB are obtained in [Bmim][TfO] and [Hmim][TfO]. In contrast, in [Emim][TfO], bonding of [TfO] to LYS-290 disrupts the interactions between LYS-290 and ILE-285, which leads to a closed catalytic gate conformation with low accessibility of substrates to the catalytic triad. In [Omim][TfO], strong hydrophobic interactions between [Omim]+ and LEU-278 result in a significant loss of the secondary structure of the α-10 helix and cause the exposure of the catalytic triad to ILs, which affects the stability of the catalytic triad and consequently deteriorates the enzyme activity. Overall, our study indicates that a high ion coordination number ([Emim][TfO]) or the presence of a long hydrophobic tail ([Omim][TfO]) can facilitate ion–protein interactions that cause structural distortions and a decrease in CALB enzyme activity in ILs.

Graphical abstract: The effect of imidazolium cations on the structure and activity of the Candida antarctica Lipase B enzyme in ionic liquids

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Supplementary files

Article information


Submitted
08 Apr 2016
Accepted
19 May 2016
First published
27 May 2016

Phys. Chem. Chem. Phys., 2016,18, 22062-22069
Article type
Paper

The effect of imidazolium cations on the structure and activity of the Candida antarctica Lipase B enzyme in ionic liquids

H. S. Kim, D. Eom, Y. Koo and Y. G. Yingling, Phys. Chem. Chem. Phys., 2016, 18, 22062
DOI: 10.1039/C6CP02355J

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