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Issue 25, 2016
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Synthesis of the rare disaccharide nigerose by structure-based design of a phosphorylase mutant with altered regioselectivity

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Abstract

In the absence of the natural acceptor inorganic phosphate wild-type sucrose phosphorylase from Bifidobacterium adolescentis (BaSP) produces maltose (4-O-α-D-glucopyranosyl-D-glucose) and kojibiose (2-O-α-D-glucopyranosyl-D-glucose) as sole transfer products. A Q345F exchange switches the enzyme's regioselectivity from 2 to 3 exclusively, yielding the rare sugar nigerose (3-O-α-D-glucopyranosyl-D-glucose, sakebiose).

Graphical abstract: Synthesis of the rare disaccharide nigerose by structure-based design of a phosphorylase mutant with altered regioselectivity

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Supplementary files

Article information


Submitted
29 Jan 2016
Accepted
09 Feb 2016
First published
10 Feb 2016

Chem. Commun., 2016,52, 4625-4627
Article type
Communication

Synthesis of the rare disaccharide nigerose by structure-based design of a phosphorylase mutant with altered regioselectivity

M. Kraus, J. Görl, M. Timm and J. Seibel, Chem. Commun., 2016, 52, 4625
DOI: 10.1039/C6CC00934D

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