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Issue 18, 2016
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Converting bulk sugars into prebiotics: semi-rational design of a transglucosylase with controlled selectivity

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Abstract

Despite the growing importance of prebiotics in nutrition and gastroenterology, their structural variety is currently still very limited. The lack of straightforward procedures to gain new products in sufficient amounts often hampers application testing and further development. Although the enzyme sucrose phosphorylase can be used to produce the rare disaccharide kojibiose (α-1,2-glucobiose) from the bulk sugars sucrose and glucose, the target compound is only a side product that is difficult to isolate. Accordingly, for this biocatalyst to become economically attractive, the formation of other glucobioses should be avoided and therefore we applied semi-rational mutagenesis and low-throughput screening, which resulted in a double mutant (L341I_Q345S) with a selectivity of 95% for kojibiose. That way, an efficient and scalable production process with a yield of 74% could be established, and with a simple yeast treatment and crystallization step over a hundred grams of highly pure kojibiose (>99.5%) was obtained.

Graphical abstract: Converting bulk sugars into prebiotics: semi-rational design of a transglucosylase with controlled selectivity

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Supplementary files

Article information


Submitted
02 Dec 2015
Accepted
03 Feb 2016
First published
03 Feb 2016

Chem. Commun., 2016,52, 3687-3689
Article type
Communication
Author version available

Converting bulk sugars into prebiotics: semi-rational design of a transglucosylase with controlled selectivity

T. Verhaeghe, K. De Winter, M. Berland, R. De Vreese, M. D'hooghe, B. Offmann and T. Desmet, Chem. Commun., 2016, 52, 3687
DOI: 10.1039/C5CC09940D

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