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Issue 97, 2016
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One-pot four-enzyme synthesis of thymidinediphosphate-l-rhamnose

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Abstract

A new, robust one-pot four-enzyme synthetic method was developed for thymidinediphosphate-L-rhamnose starting from D-glucose-1-phosphate. The enzymes, Glc-1-P thymidylyltransferase, dTDP-Glc-4,6-dehydratase, dTDP-4-keto-6-deoxy-Glc-3,5-epimerase and dTDP-4-keto-Rha reductase were derived from Streptococcus pneumonia serotype 23F, expressed in Escherichia coli, and studied in detail to provide the first direct evidence for their functions.

Graphical abstract: One-pot four-enzyme synthesis of thymidinediphosphate-l-rhamnose

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Supplementary files

Article information


Submitted
17 Oct 2016
Accepted
08 Nov 2016
First published
09 Nov 2016

Chem. Commun., 2016,52, 13995-13998
Article type
Communication

One-pot four-enzyme synthesis of thymidinediphosphate-L-rhamnose

S. Li, H. Wang, J. Ma, G. Gu, Z. Chen and Z. Guo, Chem. Commun., 2016, 52, 13995
DOI: 10.1039/C6CC08366H

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