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Issue 97, 2016
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Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher

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Abstract

Simple plug-and-playable fluorescent cell imaging modular toolkits are established using the bacterial superglue SpyTag/SpyCatcher protein ligation system. A variety of affibody–fluorescent protein conjugates (AFPCs) are post-translationally generated via the isopeptide bond formation, and each AFPC effectively recognizes and binds to its targeting cells, visualizing them with selective colors on demand.

Graphical abstract: Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher

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Publication details

The article was received on 09 Sep 2016, accepted on 09 Nov 2016 and first published on 17 Nov 2016


Article type: Communication
DOI: 10.1039/C6CC07363H
Chem. Commun., 2016,52, 14051-14054

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    Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher

    H. Moon, Y. Bae, H. Kim and S. Kang, Chem. Commun., 2016, 52, 14051
    DOI: 10.1039/C6CC07363H

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