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Issue 99, 2016
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Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein

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Abstract

A 5mC-selective TALE-repeat was created by screening a TALE repeat library containing randomized amino acids at repeat variable diresidues and their neighboring residues. The new repeat showed high 5mC discrimination ability. An artificial TALE containing the new repeat activated an endogenous gene in a genomic methylation status-dependent manner.

Graphical abstract: Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein

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Publication details

The article was received on 19 Aug 2016, accepted on 16 Nov 2016 and first published on 16 Nov 2016


Article type: Communication
DOI: 10.1039/C6CC06824C
Chem. Commun., 2016,52, 14238-14241
  • Open access: Creative Commons BY license
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    Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein

    S. Tsuji, S. Futaki and M. Imanishi, Chem. Commun., 2016, 52, 14238
    DOI: 10.1039/C6CC06824C

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