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Issue 40, 2016
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HaloTag mediated artificial cellulosome assembly on a rolling circle amplification DNA template for efficient cellulose hydrolysis

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Abstract

We report here the generation of four-component artificial cellulosomes onto a DNA scaffold using the self-labeling HaloTag for DNA conjugation. The resulting structures exhibited significantly improved cellulosome assembly as well as cellulose hydrolysis over compatible structures generated using protein scaffolds. Cellulose hydrolysis was further enhanced by 2-fold using the more complex cellulosome structures assembled onto DNA templates generated by rolling circle amplification (RCA). The flexibility to insert additional hybridization sites in a multiplexing manner using RCA should enable the assembly of a larger array of cellulases to better mimic the enzyme diversity of naturally occurring cellulosomes.

Graphical abstract: HaloTag mediated artificial cellulosome assembly on a rolling circle amplification DNA template for efficient cellulose hydrolysis

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Publication details

The article was received on 07 Mar 2016, accepted on 19 Apr 2016 and first published on 19 Apr 2016


Article type: Communication
DOI: 10.1039/C6CC02035F
Chem. Commun., 2016,52, 6701-6704

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    HaloTag mediated artificial cellulosome assembly on a rolling circle amplification DNA template for efficient cellulose hydrolysis

    Q. Sun and W. Chen, Chem. Commun., 2016, 52, 6701
    DOI: 10.1039/C6CC02035F

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