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Issue 17, 2016
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Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins

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Abstract

This work describes a general methodology for enhanced enzyme-linked immunosorbent assay (ELISA) that integrates enzyme-coated microparticle probes for robust yet highly efficient signal amplification and a handheld pH meter for a simple, portable, quantitative readout. Its utility is well demonstrated with the detection of the target protein with a 14-fold enhancement of sensitivity in comparison with the conventional optical ELISA.

Graphical abstract: Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins

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The article was received on 29 Nov 2015, accepted on 09 Dec 2015 and first published on 09 Dec 2015


Article type: Communication
DOI: 10.1039/C5CC09852A
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Chem. Commun., 2016,52, 3474-3477

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    Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins

    Y. Zhang, J. Yang, J. Nie, J. Yang, D. Gao, L. Zhang and J. Li, Chem. Commun., 2016, 52, 3474
    DOI: 10.1039/C5CC09852A

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