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Issue 3, 2016
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The application of fluorescence-conjugated pyrrole/imidazole polyamides in the characterization of protein–DNA complex formation

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Abstract

N-Methylpyrrole (Py)–N-methylimidazole-(Im) polyamides are sequence-specific DNA-binding modules that are widely used for gene regulation, as synthetic transcriptional factors and as sequence-specific DNA alkylating agents. Recently, Py–Im polyamides have been conjugated with fluorophores, resulting in conjugates that are useful for the detection of specific DNA sequences. A Förster resonance energy transfer has been observed between Cy3- and Cy5-conjugated Py–Im polyamides on the nucleosome, indicating that fluorescence-conjugated Py–Im polyamides could possibly be used to characterise protein–DNA complexes. In this minireview, we discuss recent reports regarding fluorescence-conjugated Py–Im polyamides and their future application in the characterization of protein–DNA complex formation.

Graphical abstract: The application of fluorescence-conjugated pyrrole/imidazole polyamides in the characterization of protein–DNA complex formation

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Publication details

The article was received on 30 Jun 2015, accepted on 10 Dec 2015 and first published on 06 Jan 2016


Article type: Minireview
DOI: 10.1039/C5BM00214A
Biomater. Sci., 2016,4, 391-399

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    The application of fluorescence-conjugated pyrrole/imidazole polyamides in the characterization of protein–DNA complex formation

    Y. Han, H. Sugiyama and Y. Harada, Biomater. Sci., 2016, 4, 391
    DOI: 10.1039/C5BM00214A

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