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Issue 26, 2016
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Determination of α-tocopherol in cereal grains by use of saponification coupled with ionic-liquid-based dispersive liquid–liquid microextraction before high-performance liquid chromatography-mass spectrometry

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Abstract

A selective and low organic-solvent-consuming method of sample preparation combined with high-performance liquid chromatography-mass spectrometry detection is introduced for the analysis of α-tocopherol in cereal grains. The sample treatment involves initial saponification and preconcentration of the analyte performed by ionic-liquid-based dispersive liquid–liquid microextraction, with ethanol as disperser solvent and 1-octyl-3-methylimidazolium hexafluorophosphate as extraction solvent. The method offered excellent linearity over a range of 0.5–15 μg g−1 with a correlation coefficient of r > 0.998. Intra-day and inter-day repeatability, expressed as relative standard deviation were 3.4% and 5.9%, respectively. The quantitation limits and detection limits were 2.5 and 0.7 ng g−1. Satisfactory recoveries ranging from 86.5% to 106.3% were obtained. The developed method was successfully applied to 12 cereal grain samples.

Graphical abstract: Determination of α-tocopherol in cereal grains by use of saponification coupled with ionic-liquid-based dispersive liquid–liquid microextraction before high-performance liquid chromatography-mass spectrometry

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Publication details

The article was received on 14 Apr 2016, accepted on 04 Jun 2016 and first published on 06 Jun 2016


Article type: Paper
DOI: 10.1039/C6AY01112H
Anal. Methods, 2016,8, 5283-5287

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    Determination of α-tocopherol in cereal grains by use of saponification coupled with ionic-liquid-based dispersive liquid–liquid microextraction before high-performance liquid chromatography-mass spectrometry

    Q. Xie, D. Sun, J. Cao, L. Jia, B. Hou and D. Li, Anal. Methods, 2016, 8, 5283
    DOI: 10.1039/C6AY01112H

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