Jump to main content
Jump to site search

Issue 16, 2016
Previous Article Next Article

Simultaneous determination of 14 components in Shuanghua Baihe tablets by ultrasonic-assisted extraction and HPLC-MS/MS

Author affiliations

Abstract

A simple, sensitive, selective, and reliable high-performance liquid chromatograph-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the simultaneous detection and quantification of 14 compounds. These compounds were separated completely within 13 min on a Kinetex C18 core–shell column with acetonitrile and 0.1% formic acid as the mobile phase. The column was coupled to an ESI-triple-quadrupole mass spectrometer operated in positive and negative ion mode simultaneously. In total, 14 components were quantified in multiple reactions monitoring acquisition mode. The limits of detection of these compounds ranged from 0.12 to 2.10 ng mL−1, whereas the limits of quantitation ranged from 0.40 to 7.09 ng mL−1. All the calibration curves showed good linearities (r > 0.999) within the test ranges. An orthogonal design experiment was also used to investigate the effect of the extraction variables, including temperature, methanol to water ratio percent, and sonication time, on the ultrasound-assisted extraction (UAE). The optimum extraction condition and validated method was successfully used to analyze 10 batches of Shuanghua Baihe tablets (SBT) bought from Chinese markets. The method was validated to be simple, rapid, accurate and reliable to simultaneously determine 14 components in SBT and our results provide a new basis for the overall assessment of SBT quality.

Graphical abstract: Simultaneous determination of 14 components in Shuanghua Baihe tablets by ultrasonic-assisted extraction and HPLC-MS/MS

Back to tab navigation

Article information


Submitted
16 Jan 2016
Accepted
22 Mar 2016
First published
23 Mar 2016

Anal. Methods, 2016,8, 3359-3365
Article type
Paper

Simultaneous determination of 14 components in Shuanghua Baihe tablets by ultrasonic-assisted extraction and HPLC-MS/MS

L. Zheng, X. Huo, C. Wang, H. Cong, T. Xiang, B. Wu, B. Zhang, S. Huang, L. Zhang and X. Ma, Anal. Methods, 2016, 8, 3359
DOI: 10.1039/C6AY00143B

Social activity

Search articles by author

Spotlight

Advertisements