Simultaneous preparation and characterization of three high-purity type B fumonisins from maize culture†
A reliable and large-scale preparative method was developed for the simultaneous preparation of high-purity fumonisin B1 (FB1), fumonisin B2 (FB2) and fumonisin B3 (FB3) from maize culture inoculated with Fusarium moniliforme in this study. The targeted B-series fumonisins (FBs) were firstly extracted with acetonitrile/water (50 : 50, v/v) and then purified using MAX cartridges. The subsequent chromatographic preparative procedures on a Unitary C18 column (250 mm × 10 mm, 5 μm) and a SB-CN column (250 mm × 9.4 mm, 5 μm) were performed to effectively eliminate the other interferents to obtain the high-purity compounds. The prepared compounds were clearly identified to be FB1, FB2 and FB3 using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR). The purities were 96.25 ± 0.18%, 98.07 ± 0.18% and 97.36 ± 0.74% for FB1, FB2 and FB3, respectively, which were determined by the external standard LC-MS/MS method. Within 72 h, large amounts of high-purity FB1 (80 mg), FB2 (16 mg) and FB3 (16 mg) could be simultaneously prepared by the established approach.