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Issue 6, 2016
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Detecting carbohydrate–lectin interactions using a fluorescent probe based on DBD dyes

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Abstract

Herein we present an efficient synthesis of a biomimetic probe with modular construction that can be specifically bound by the mannose binding FimH protein – a surface adhesion protein of E. coli bacteria. The synthesis combines the new and interesting DBD dye with the carbohydrate ligand mannose via a Click reaction. We demonstrate the binding to E. coli bacteria over a large concentration range and also present some special characteristics of those molecules that are of particular interest for the application as a biosensor. In particular, the mix-and-measure ability and the very good photo-stability should be highlighted here.

Graphical abstract: Detecting carbohydrate–lectin interactions using a fluorescent probe based on DBD dyes

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Publication details

The article was received on 14 Nov 2015, accepted on 13 Jan 2016 and first published on 14 Jan 2016


Article type: Communication
DOI: 10.1039/C5AY02991K
Anal. Methods, 2016,8, 1235-1238
  • Open access: Creative Commons BY license
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    Detecting carbohydrate–lectin interactions using a fluorescent probe based on DBD dyes

    D. Bader, D. T. Klier, C. Hettrich, F. F. Bier and P. Wessig, Anal. Methods, 2016, 8, 1235
    DOI: 10.1039/C5AY02991K

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