Issue 8, 2016
From the journal:

Analyst

Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry†

Cunyu Yan,‡a   Jason W. Schmidberger,§‡a   Fabio Parmeggiani,a   Shaneela A. Hussain,a   Nicholas J. Turner,a   Sabine L. Flitsch*a  and  Perdita Barran*a  

* Corresponding authors

a School of Chemistry, Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
E-mail: perdita.barran@manchester.ac.uk, sabine.flitsch@manchester.ac.uk

Abstract

The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and μL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.

Graphical abstract: Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

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Article information

DOI
https://doi.org/10.1039/C6AN00617E
Article type
Communication
Submitted
15 Mar 2016
Accepted
15 Mar 2016
First published
15 Mar 2016

Analyst, 2016,141, 2351-2355

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Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

C. Yan, J. W. Schmidberger, F. Parmeggiani, S. A. Hussain, N. J. Turner, S. L. Flitsch and P. Barran, Analyst, 2016, 141, 2351 DOI: 10.1039/C6AN00617E

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