Jump to main content
Jump to site search

Issue 4, 2016
Previous Article Next Article

Accurate biometal quantification per individual Caenorhabditis elegans

Author affiliations

Abstract

In the life sciences, small model-organisms are an established research platform. Due to the economy of culturing and maintenance animals such as the roundworm Caenorhabditis elegans, and the fly Drosophila melanogaster, have been instrumental for investigating key genetic pathways, early development, neuronal function, as well as disease pathogenesis and toxicology. Small model organisms have also found utility in the study of inorganic biochemistry, where the role of metal ion cofactors are investigated for numerous fundamental cellular processes. The metabolism and homeostasis of metal ions is also central to many aspects of biology and disease. Accurate quantification of endogenous metal ion content is an important determinant for many biological questions. There is currently no standardised method for quantifying biometal content in individual C. elegans or estimating the variation between individuals within clonal populations. Here, we have determined that ten or more adults are required to quantify physiologically important metals via inductively coupled plasma mass spectrometry (ICP-MS). The accuracy and precision of this method was then compared to synchrotron-based X-ray fluorescence microscopy (XFM) to determine the variation between isogenic, developmentally synchronous C. elegans adults.

Graphical abstract: Accurate biometal quantification per individual Caenorhabditis elegans

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Dec 2015, accepted on 19 Jan 2016 and first published on 26 Jan 2016


Article type: Paper
DOI: 10.1039/C5AN02544C
Analyst, 2016,141, 1434-1439
  • Open access: Creative Commons BY license
  •   Request permissions

    Accurate biometal quantification per individual Caenorhabditis elegans

    K. Ganio, S. A. James, D. J. Hare, B. R. Roberts and G. McColl, Analyst, 2016, 141, 1434
    DOI: 10.1039/C5AN02544C

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements