Radiolanthanide-loaded agglomerated Fe3O4 nanoparticles for possible use in the treatment of arthritis: formulation, characterization and evaluation in rats

Abstract

This investigation reports the preparation of agglomerated Fe₃O₄ nanoparticles and evaluation of its utility as a viable carrier in the preparation of radiolanthanides as potential therapeutic agents for the treatment of arthritis. The material was synthesized by a chemical route and characterized by XRD, FT-IR, SEM, EDX and TEM analysis. The surface of agglomerated particle possessed ion pairs (–O⁻:Na⁺) after dispersing particles in a NaHCO₃ solution at pH = 7 which is conducive for radiolanthanide (*Ln = ⁹⁰Y, ¹⁵³Sm, ¹⁶⁶Ho, ¹⁶⁹Er, ¹⁷⁷Lu) loading by replacement of Na⁺ ions with tripositive radiolanthanide ions. Radiolanthanide-loaded particulates exhibited excellent in vitro stability up to ∼3 half-lives of the respective lanthanide radionuclides when stored in normal saline at 37 °C. The radiochemical purities of the loaded particulates were found to be retained to the extent of >70% after 48 h of storage when challenged by a strong chelator DTPA present at a concentration as high as 5 mM, indicating fairly strong chemical association of lanthanides with agglomerated Fe₃O₄ nanoparticles. Biodistribution studies of ⁹⁰Y and ¹⁶⁶Ho-loaded particulates carried out after intra-articular injection into one of the knee joints of a normal Wistar rat revealed near-complete retention of the radioactive preparations (>98% of the administered radioactivity) within the joint cavity even after 72 h post injection. This was further confirmed by sequential whole-body radio-luminescence imaging. These experimental results are indicative of the potential use of radiolanthanide-loaded agglomerated Fe₃O₄ nanoparticles for the treatment of arthritis.