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Issue 29, 2015
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Intracellular delivery of BSA by phosphonate@silica nanoparticles

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Abstract

Intracellular protein (BSA) delivery by a phosphonate@mesoporous silica nanoparticle vehicle, PMSN, with high load capacity for the relatively large test protein BSA, is described. Wide pore (11.6 nm) PMSN nanoparticles were synthesised and loaded with a BSA cargo to give BSA#@PMSN*, where # and * signify Fluorescein and Rhodamine fluorescent labels respectively. Internalisation of BSA#@PMSN*s by HeLa cells was analysed from confocal microscopy and TEM images after dose and time dependent treatments. No evidence of cytotoxicity was observed after 24 h and in contrast to PMSN* no significant loss of BSA#@PMSN* was observed after 3 h incubation of the loaded cells in DMEM. Receptor blocking experiments showed caveolar uptake of PMSN* and folate receptor mediated uptake of BSA#@PMSN*s.

Graphical abstract: Intracellular delivery of BSA by phosphonate@silica nanoparticles

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Publication details

The article was received on 26 Mar 2015, accepted on 14 Jun 2015 and first published on 29 Jun 2015


Article type: Paper
DOI: 10.1039/C5TB00555H
J. Mater. Chem. B, 2015,3, 6057-6070

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    Intracellular delivery of BSA by phosphonate@silica nanoparticles

    S. P. Maddala, G. Mastroianni, D. Velluto and A. C. Sullivan, J. Mater. Chem. B, 2015, 3, 6057
    DOI: 10.1039/C5TB00555H

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