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Issue 26, 2015
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An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

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Abstract

An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.

Graphical abstract: An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

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Supplementary files

Article information


Submitted
25 Mar 2015
Accepted
05 Jun 2015
First published
08 Jun 2015

J. Mater. Chem. B, 2015,3, 5166-5171
Article type
Communication
Author version available

An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

C. C. Mayorga-Martinez, A. Chamorro-García, L. Serrano, L. Rivas, D. Quesada-Gonzalez, L. Altet, O. Francino, A. Sánchez and A. Merkoçi, J. Mater. Chem. B, 2015, 3, 5166
DOI: 10.1039/C5TB00545K

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