Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity†
To facilitate automated real-time monitoring of the formation or disruption of an epithelial monolayer, phase angle spectroscopy was performed in custom-designed cell culture dishes including a transparent conductive polymer-based 2-electrode device. A peak in φ amplitude at 103–105 Hz specifically correlated to increased capacitance by proliferating cells. Dynamic monitoring of cell density, and barrier integrity during monolayer formation and disruption were investigated in combination with live-cell microscopy.
- This article is part of the themed collection: Bioelectronics