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Issue 25, 2015
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Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

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Abstract

To facilitate automated real-time monitoring of the formation or disruption of an epithelial monolayer, phase angle spectroscopy was performed in custom-designed cell culture dishes including a transparent conductive polymer-based 2-electrode device. A peak in φ amplitude at 103–105 Hz specifically correlated to increased capacitance by proliferating cells. Dynamic monitoring of cell density, and barrier integrity during monolayer formation and disruption were investigated in combination with live-cell microscopy.

Graphical abstract: Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

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Supplementary files

Article information


Submitted
26 Feb 2015
Accepted
26 Apr 2015
First published
27 Apr 2015

This article is Open Access

J. Mater. Chem. B, 2015,3, 4997-5000
Article type
Communication
Author version available

Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

S. Löffler and A. Richter-Dahlfors, J. Mater. Chem. B, 2015, 3, 4997
DOI: 10.1039/C5TB00381D

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