Jump to main content
Jump to site search

Issue 8, 2015
Previous Article Next Article

Design of multi-functional linear polymers that capture and neutralize a toxic peptide: a comparison with cross-linked nanoparticles

Author affiliations

Abstract

In this paper, a library of multi-functional linear poly-N-isopropylacrylamide (pNIPAm) polymers having a range of molecular weights and functional groups were synthesized and their interaction with the hemolytic peptide, melittin, was examined. The linear pNIPAm (LPs) containing both tert-butyl group and carboxylic acids bound with the peptide by a combination of hydrophobic and electrostatic interactions and neutralized its toxicity. The melittin binding capacity and affinity of each LP was quantified and further compared with cross-linked multi-functional nanogel particles (NPs) having same combination of functional groups. The binding capacity of the LPs (weight of captured melittin/weight of LP) was independent of their molecular weight and was three times higher than that of previously reported NPs. The binding constant depended on the molecular weight of the LPs, showing the highest value of 1.1 × 108 (M−1) for a ∼1000 mer linear polymer with 40% tert-butyl group and 20% carboxylic acid. Comparison of the interactions of the LPs and NPs suggested the importance of the flexibility of the polymer chain in order to achieve high binding capacity and affinity.

Graphical abstract: Design of multi-functional linear polymers that capture and neutralize a toxic peptide: a comparison with cross-linked nanoparticles

Back to tab navigation

Supplementary files

Publication details

The article was received on 27 Nov 2014, accepted on 15 Jan 2015 and first published on 16 Jan 2015


Article type: Paper
DOI: 10.1039/C4TB01967A
Author version
available:
Download author version (PDF)
J. Mater. Chem. B, 2015,3, 1706-1711

  •   Request permissions

    Design of multi-functional linear polymers that capture and neutralize a toxic peptide: a comparison with cross-linked nanoparticles

    Y. Wada, H. Lee, Y. Hoshino, S. Kotani, K. J. Shea and Y. Miura, J. Mater. Chem. B, 2015, 3, 1706
    DOI: 10.1039/C4TB01967A

Search articles by author

Spotlight

Advertisements