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Issue 12, 2015

Target-fueled DNA walker for highly selective miRNA detection

Author affiliations

Abstract

Artificial DNA motifs as architectural scaffolds have been widely used to assemble a variety of nanoscale devices. Synthetic DNA nanostructures have accomplished mechanical switching in response to external stimuli, suggesting the promise of constructing a walking device that is being used in the field of biosensors. Here, we design a novel miRNA-responsive DNA walker biosensor based on strand displacement cascades and an enzymatic recycling cleavage strategy. By using miRNA as a driving force, the DNA walkers can be activated to move along the track and generate specific signals for let-7a with a high signal-to-noise ratio. This biosensor exhibits excellent analytical performance toward the sensing of let-7a with great specificity for resolving one nucleotide variation and a detection limit of 58 fM. Such an ultraselective sensor shows that DNA nanostructures have great potential in providing platforms for applications in the fields of biosensing, clinical diagnostics and environmental sample analysis.

Graphical abstract: Target-fueled DNA walker for highly selective miRNA detection

Supplementary files

Article information


Submitted
29 Jul 2015
Accepted
10 Sep 2015
First published
10 Sep 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2015,6, 6777-6782
Article type
Edge Article

Target-fueled DNA walker for highly selective miRNA detection

L. Wang, R. Deng and J. Li, Chem. Sci., 2015, 6, 6777 DOI: 10.1039/C5SC02784E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.


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