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Issue 11, 2015
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Cell paintballing using optically targeted coacervate microdroplets

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Abstract

We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet–membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel “cell paintballing” technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.

Graphical abstract: Cell paintballing using optically targeted coacervate microdroplets

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Supplementary files

Article information


Submitted
23 Jun 2015
Accepted
20 Jul 2015
First published
20 Jul 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2015,6, 6106-6111
Article type
Edge Article
Author version available

Cell paintballing using optically targeted coacervate microdroplets

J. P. K. Armstrong, S. N. Olof, M. D. Jakimowicz, A. P. Hollander, S. Mann, S. A. Davis, M. J. Miles, A. J. Patil and A. W. Perriman, Chem. Sci., 2015, 6, 6106
DOI: 10.1039/C5SC02266E

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