Issue 11, 2015

Cell paintballing using optically targeted coacervate microdroplets


We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet–membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel “cell paintballing” technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.

Graphical abstract: Cell paintballing using optically targeted coacervate microdroplets

Supplementary files

Article information

Article type
Edge Article
23 Jun 2015
20 Jul 2015
First published
20 Jul 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2015,6, 6106-6111

Author version available

Cell paintballing using optically targeted coacervate microdroplets

J. P. K. Armstrong, S. N. Olof, M. D. Jakimowicz, A. P. Hollander, S. Mann, S. A. Davis, M. J. Miles, A. J. Patil and A. W. Perriman, Chem. Sci., 2015, 6, 6106 DOI: 10.1039/C5SC02266E

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