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Issue 10, 2015
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Visualising mouse neuroanatomy and function by metal distribution using laser ablation-inductively coupled plasma-mass spectrometry imaging

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Abstract

Metals have a number of important roles within the brain. We used laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to map the three-dimensional concentrations and distributions of transition metals, in particular iron (Fe), copper (Cu) and zinc (Zn) within the murine brain. LA-ICP-MS is one of the leading analytical tools for measuring metals in tissue samples. Here, we present a complete data reduction protocol for measuring metals in biological samples, including the application of a pyramidal voxel registration technique to reproducibly align tissue sections. We used gold (Au) nanoparticle and ytterbium (Yb)-tagged tyrosine hydroxylase antibodies to assess the co-localisation of Fe and dopamine throughout the entire mouse brain. We also examined the natural clustering of metal concentrations within the murine brain to elucidate areas of similar composition. This clustering technique uses a mathematical approach to identify multiple ‘elemental clusters’, avoiding user bias and showing that metal composition follows a hierarchical organisation of neuroanatomical structures. This work provides new insight into the distinct compartmentalisation of metals in the brain, and presents new avenues of exploration with regard to region-specific, metal-associated neurodegeneration observed in several chronic neurodegenerative diseases.

Graphical abstract: Visualising mouse neuroanatomy and function by metal distribution using laser ablation-inductively coupled plasma-mass spectrometry imaging

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Publication details

The article was received on 19 Jun 2015, accepted on 27 Jul 2015 and first published on 27 Jul 2015


Article type: Edge Article
DOI: 10.1039/C5SC02231B
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Chem. Sci., 2015,6, 5383-5393
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    Visualising mouse neuroanatomy and function by metal distribution using laser ablation-inductively coupled plasma-mass spectrometry imaging

    B. Paul, D. J. Hare, D. P. Bishop, C. Paton, V. T. Nguyen, N. Cole, M. M. Niedwiecki, E. Andreozzi, A. Vais, J. L. Billings, L. Bray, A. I. Bush, G. McColl, B. R. Roberts, P. A. Adlard, D. I. Finkelstein, J. Hellstrom, J. M. Hergt, J. D. Woodhead and P. A. Doble, Chem. Sci., 2015, 6, 5383
    DOI: 10.1039/C5SC02231B

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