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Issue 10, 2015
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Constructing real-time, wash-free, and reiterative sensors for cell surface proteins using binding-induced dynamic DNA assembly

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Abstract

Cell surface proteins are an important class of biomarkers for fundamental biological research and for disease diagnostics and treatment. In this communication, we report a universal strategy to construct sensors that can achieve rapid imaging of cell surface proteins without any separation by using binding-induced dynamic DNA assembly. As a proof-of-principle, we developed a real-time and wash-free sensor for an important breast cancer biomarker, human epidermal growth factor receptor-2 (HER2). We then demonstrated that this sensor could be used for imaging and sensing HER2 on both fixed and live breast cancer cells. Additionally, we have also incorporated toehold-mediated DNA strand displacement reactions into the HER2 sensor, which allows for reiterating (switching on/off) fluorescence signals for HER2 from breast cancer cells in real-time.

Graphical abstract: Constructing real-time, wash-free, and reiterative sensors for cell surface proteins using binding-induced dynamic DNA assembly

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Publication details

The article was received on 24 May 2015, accepted on 07 Jul 2015 and first published on 08 Jul 2015


Article type: Edge Article
DOI: 10.1039/C5SC01870F
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Citation: Chem. Sci., 2015,6, 5729-5733
  • Open access: Creative Commons BY-NC license
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    Constructing real-time, wash-free, and reiterative sensors for cell surface proteins using binding-induced dynamic DNA assembly

    Y. Tang, Z. Wang, X. Yang, J. Chen, L. Liu, W. Zhao, X. C. Le and F. Li, Chem. Sci., 2015, 6, 5729
    DOI: 10.1039/C5SC01870F

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