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Issue 7, 2015

Luminescence switch-on detection of protein tyrosine kinase-7 using a G-quadruplex-selective probe

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Abstract

A series of luminescent iridium(III) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The iridium(III) complex 9 [Ir(pbi)2(5,5-dmbpy)]PF6 (where pbi = 2-phenyl-1H-benzo[d]imidazole; 5,5-dmbpy = 5,5′-dimethyl-2,2′-bipyridine) exhibited high luminescence for G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a G-quadruplex-based assay for protein tyrosine kinase-7 (PTK7) in aqueous solution. PTK7 is an important biomarker for a range of leukemias and solid tumors. In the presence of PTK7, the specific binding of the sgc8 aptamer sequence triggers a structural transition and releases the G-quadruplex-forming sequence. The formation of the nascent G-quadruplex structure is then detected by the G-quadruplex-selective iridium(III) complex with an enhanced luminescent response. Moreover, the application of the assay for detecting PTK7 in cellular debris and membrane protein extract was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for PTK7.

Graphical abstract: Luminescence switch-on detection of protein tyrosine kinase-7 using a G-quadruplex-selective probe

Supplementary files

Article information


Submitted
13 Apr 2015
Accepted
17 May 2015
First published
18 May 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2015,6, 4284-4290
Article type
Edge Article
Author version available

Luminescence switch-on detection of protein tyrosine kinase-7 using a G-quadruplex-selective probe

S. Lin, W. Gao, Z. Tian, C. Yang, L. Lu, J. Mergny, C. Leung and D. Ma, Chem. Sci., 2015, 6, 4284 DOI: 10.1039/C5SC01320H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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