Issue 106, 2015

Sensitive fluorescence “turn-on” detection of bleomycin based on a superquenched perylene–DNA complex

Abstract

Bleomycins (BLMs) are widely used in the clinical treatment of various cancers. Here, taking advantage of the super-quenching effect of the cationic perylene diimide derivative (PDI) on adjacent fluorophores, we reported a PDI–DNA complex based method for fluorescence “turn-on” detection of BLM, based on BLM–Fe(II) mediated DNA strand scission. Owing to the very high quenching efficiency of aggregated PDI, the method shows low background fluorescence and high sensitivity with a detection limit of 0.2 nM. It also exhibits a good performance in complex biological samples. Moreover, this method has the advantages of no quencher labelling cost, easy preparation and rapid response, making it a promising alternative for the determination of trace amounts of BLMs in clinical samples.

Graphical abstract: Sensitive fluorescence “turn-on” detection of bleomycin based on a superquenched perylene–DNA complex

Supplementary files

Article information

Article type
Paper
Submitted
07 Sep 2015
Accepted
06 Oct 2015
First published
08 Oct 2015

RSC Adv., 2015,5, 86849-86854

Author version available

Sensitive fluorescence “turn-on” detection of bleomycin based on a superquenched perylene–DNA complex

R. Kong, N. Sun, F. Qu, H. Wu, H. Wang and J. You, RSC Adv., 2015, 5, 86849 DOI: 10.1039/C5RA18227A

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