Immobilization of laccase on magnetic silica nanoparticles and its application in the oxidation of guaiacol, a phenolic lignin model compound
Abstract
A novel magnetically separable laccase immobilized system was constructed by immobilizing Aspergillus laccase on the core–shell structure of amino-functionalised magnetic silica nanoparticles (AF-MSNPs) via a chemical crosslinking method. A large immobilization capacity (613.5 ± 10.5 mg g−1), activity recovery of 53.4 ± 3.1% and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability, storage stability and operational stability were increased to a great extent. The application of the immobilized system in catalyzing the guaiacol as a phenolic lignin model compound was investigated. The high catalytic efficiency for guaiacol indicated that utilizing immobilized laccase to catalyse lignin is a very promising technology for further applications in the wood industry.