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Issue 90, 2015
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A theranostic polycation containing trehalose and lanthanide chelate domains for siRNA delivery and monitoring

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Abstract

A trehalose-based polycation that contains lanthanide-chelate domains has been examined as a theranostic vehicle for siRNA delivery. By chelating the polymers with gadolinium and terbium ions, the polymers offer magnetic resonance and luminescence-based observation properties. Lanthanide resonance energy transfer (LRET) studies were examined with the Tb3+-chelated polymers (LRET donor) to monitor polyplex association with the LRET acceptor tetramethylrhodamine (TMR)-labeled siRNA. The polyplex formulations were compared to structures that do not contain trehalose within the polymer backbone and it was found that the presence of trehalose appears to increase siRNA delivery and gene knockdown efficiency in cultured glioblastoma cells (U87) that stably express luciferase. This study thus provides a preliminary theranostic polymer design model for delivery and monitoring of siRNA delivery.

Graphical abstract: A theranostic polycation containing trehalose and lanthanide chelate domains for siRNA delivery and monitoring

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Supplementary files

Article information


Submitted
27 Jul 2015
Accepted
20 Aug 2015
First published
03 Sep 2015

RSC Adv., 2015,5, 74102-74106
Article type
Communication
Author version available

A theranostic polycation containing trehalose and lanthanide chelate domains for siRNA delivery and monitoring

L. Xue, S. S. Kelkar, X. Wang, J. Ma, L. A. Madsen and T. M. Reineke, RSC Adv., 2015, 5, 74102
DOI: 10.1039/C5RA14325J

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