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Issue 98, 2015
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Biohybrid hematopoietic niche for expansion of hematopoietic stem/progenitor cells by using geometrically controlled fibrous layers

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Abstract

The expansion of hematopoietic stem/progenitor cells (HSPCs), which can give rise to all types of mature blood cells, is one of the actual challenges of regenerative medicine. In this study, we propose a construction of a 3D hematopoietic niche for the coculture of HSPCs and mesenchymal stem cells, which was prepared from geometrically controlled electrospun fibrous layers. The proliferation activity, retention of CD34+CD38 immunophenotype and multipotency of the HSPCs in the artificial hematopoietic niche were analyzed by FACS and colony-forming unit assay. Results showed that the 3D biohybrid hematopoietic niche has a capacity to support self-renewal of HSPCs, maintaining their primitive phenotype and accommodate a large number of expanded cells. The geometry of the artificial niche served as a physical cue that controlled chemical signals to facilitate successful in vitro expansion and retention of HSPCs potential to differentiate into various lineages.

Graphical abstract: Biohybrid hematopoietic niche for expansion of hematopoietic stem/progenitor cells by using geometrically controlled fibrous layers

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Publication details

The article was received on 08 Jul 2015, accepted on 16 Sep 2015 and first published on 16 Sep 2015


Article type: Paper
DOI: 10.1039/C5RA13332G
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Citation: RSC Adv., 2015,5, 80357-80364

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    Biohybrid hematopoietic niche for expansion of hematopoietic stem/progenitor cells by using geometrically controlled fibrous layers

    O. Batnyam, H. Shimizu, K. Saito, T. Ishida, S. Suye and S. Fujita, RSC Adv., 2015, 5, 80357
    DOI: 10.1039/C5RA13332G

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