Jump to main content
Jump to site search

Issue 87, 2015
Previous Article Next Article

Electrocatalytic cycle of P450 cytochromes: the protective and stimulating roles of antioxidants

Author affiliations

Abstract

The electrochemical activity of cytochrome P450 (CYP) 3A4, 2C9 and 2D6 on a screen-printed graphite electrode nanostructured with gold nanoparticles and stabilized with didodecyldimethylammonium bromide was examined. The analysis of the CYP catalytic activity was carried out using a variety of electrochemical techniques, such as cyclic voltammetry, square wave voltammetry and amperometry. A sensitive electrochemical CYP-sensor system was proposed as a reliable candidate for investigating the influence of medicinal preparations – ethoxidol (2-ethyl-6-methyl-3-hydroxypyridine malate), mexidol (2-ethyl-6-methyl-3-hydroxypyridine succinate), cytochrome c and L-carnitine – on the CYP redox behavior and electrocatalytic activity. In the presence of antioxidants, the enhancement of the electrochemical signal of CYP was registered. It was shown that ethoxidol, in the concentration range of 90–600 μM, stimulates the electrochemical reduction of P450 cytochromes, with the maximum stimulating effects for CYP3A4, CYP2C9 and CYP2D6 being estimated as 181 ± 15%, 240 ± 7% and 110 ± 4% (at 540 μM ethoxidol), respectively.

Graphical abstract: Electrocatalytic cycle of P450 cytochromes: the protective and stimulating roles of antioxidants

Back to tab navigation

Publication details

The article was received on 03 Jun 2015, accepted on 10 Aug 2015 and first published on 11 Aug 2015


Article type: Paper
DOI: 10.1039/C5RA09998F
Author version
available:
Download author version (PDF)
Citation: RSC Adv., 2015,5, 71306-71313
  •   Request permissions

    Electrocatalytic cycle of P450 cytochromes: the protective and stimulating roles of antioxidants

    V. V. Shumyantseva, A. A. Makhova, T. V. Bulko, A. V. Kuzikov, E. V. Shich, V. Kukes and A. I. Archakov, RSC Adv., 2015, 5, 71306
    DOI: 10.1039/C5RA09998F

Search articles by author

Spotlight

Advertisements