A turn-on and colorimetric metal-free long lifetime fluorescent probe and its application for time-resolved luminescent detection and bioimaging of cysteine†
Abstract
A turn-on and colorimetric metal-free long lifetime fluorescent probe for sensing Cys has been synthesized. Utilizing the long emission-lifetime of DCF-MPYM, the time-resolved luminescent assay of DCF-MPYM-thiol for sensing Cys was realized successfully. DCF-MPYM-thiol can realize confocal and time-resolved microscopy bioimaging for Cys in living cells.