Jump to main content
Jump to site search

Issue 38, 2015
Previous Article Next Article

Photo-assisted inkjet printing of antibodies onto cellulose for the eco2-friendly preparation of immunoassay membranes

Author affiliations

Abstract

The current global issues have stimulated the search for both ecologically and economically friendly (eco2-friendly) materials and processes. As an affordable biopolymer, cellulose is an ideal material for developing diagnostic devices. Recently, paper-based bioanalytical devices have trended towards three-dimensional microfluidic platforms allowing multiplex diagnosis. This technological development now challenges the production process of those devices. In this perspective, the biomolecule immobilization process presented here combines an inkjet printing dispensing method with a photolinker-free photografting procedure. While many printing cycles are usually achieved to get efficient immune answers, only one to five printing passes were sufficient in this study, thereby enabling to save bio-inks. Antibodies have been successfully printed and immobilized onto paper sheets. These membranes were further used to perform lateral flow immunoassays. The visual detection limits observed were identical to those usually displayed by the classical dispensing method, regardless of the membrane material. Thus, the process developed herein is simple, time and cost-saving as well as environmentally friendly. More generally, it is a powerful tool for robust and abundant immobilization of chemical-sensitive proteins onto various cellulose-based papers and according to complex designs.

Graphical abstract: Photo-assisted inkjet printing of antibodies onto cellulose for the eco2-friendly preparation of immunoassay membranes

Back to tab navigation

Publication details

The article was received on 25 Feb 2015, accepted on 17 Mar 2015 and first published on 20 Mar 2015


Article type: Paper
DOI: 10.1039/C5RA03442F
Author version
available:
Download author version (PDF)
Citation: RSC Adv., 2015,5, 29786-29798

  •   Request permissions

    Photo-assisted inkjet printing of antibodies onto cellulose for the eco2-friendly preparation of immunoassay membranes

    J. Credou, R. Faddoul and T. Berthelot, RSC Adv., 2015, 5, 29786
    DOI: 10.1039/C5RA03442F

Search articles by author

Spotlight

Advertisements