Hydrolysis of cellobiose to monosaccharide catalyzed by functional Lanthanum(iii) metallomicelle

Abstract

A novel surfactant, 3-(dodecylimino)butan-2-one-oxime (DMBO), was synthesized. The metallomicelle La(DMBO)₂ was prepared and used as a mimic of β-glucosidase to catalyze the hydrolysis of cellobiose in weakly alkaline aqueous solution at relative low temperature (80–110 °C). This study indicated that the functional metallomicelle displayed effective catalytic activity for hydrolysis of cellobiose to monosaccharide (glucose, fructose and 1,6-anhydroglucose) and glucosyl-erythrose. The conversion of cellobiose and selectivity of monosaccharide could reach 38.5% and 71.1%, respectively, for a reaction time of 10 h at pH 9.0 and 95 °C. The possible reaction pathways of cellobiose hydrolysis are proposed and the catalysis reaction rate constant k_cat and Michaelis constant K_m for the cellobiose hydrolysis were calculated. The apparent activation energy (E_a = 84.6 kJ mol⁻¹) of cellobiose to monosaccharide was evaluated.