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Issue 34, 2015
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Dynamic constitutional frameworks (DCFs) as nanovectors for cellular delivery of DNA

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Abstract

We introduce Dynamic Constitutional Frameworks (DCFs), macromolecular structures that efficiently bind and transfect double stranded DNA. DCFs are easily synthesizable adaptive 3D networks consisting of core connection centres reversibly linked via labile imine bonds both to linear polyethyleneglycol (PEG, ∼1500 Da) and to branched polyethyleneimine (bPEI, ∼800 Da). DCFs bind linear and plasmid DNA, forming particulate polyplexes of 40–200 nm in diameter. The polyplexes are stable during gel electrophoresis, well tolerated by cells in culture, and exhibit significant transfection activity. We show that an optimal balance of PEG and bPEI components is important for building DCFs that are non-toxic and exhibit good cellular transfection activity. Our study demonstrates the versatility and effectiveness of DCFs as promising new vectors for DNA delivery.

Graphical abstract: Dynamic constitutional frameworks (DCFs) as nanovectors for cellular delivery of DNA

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Supplementary files

Article information


Submitted
27 Jun 2015
Accepted
08 Jul 2015
First published
08 Jul 2015

Org. Biomol. Chem., 2015,13, 9005-9011
Article type
Paper
Author version available

Dynamic constitutional frameworks (DCFs) as nanovectors for cellular delivery of DNA

I. Turin-Moleavin, F. Doroftei, A. Coroaba, D. Peptanariu, M. Pinteala, A. Salic and M. Barboiu, Org. Biomol. Chem., 2015, 13, 9005
DOI: 10.1039/C5OB01315A

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