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Issue 6, 2015
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A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide

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Abstract

Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investigated for the BODIPY-luminol dyad was found to be 64% resulting in a dual emissive response. Successful in cellulo biochemiluminescence via CRET was achieved in PMA activated splenocytes.

Graphical abstract: A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide

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Publication details

The article was received on 16 Nov 2014, accepted on 02 Dec 2014 and first published on 02 Dec 2014


Article type: Paper
DOI: 10.1039/C4OB02413C
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Citation: Org. Biomol. Chem., 2015,13, 1763-1767

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    A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide

    S. Bag, J.-C. Tseng and J. Rochford, Org. Biomol. Chem., 2015, 13, 1763
    DOI: 10.1039/C4OB02413C

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