Jump to main content
Jump to site search

Issue 26, 2015
Previous Article Next Article

The influence of flow, shear stress and adhesion molecule targeting on gold nanoparticle uptake in human endothelial cells

Author affiliations

Abstract

The uptake of nanoparticles by endothelial cells is dependent on shear stress adaptation and flow exposure conditions. Adaptation of primary human umbilical vein endothelial cells (HUVECs) to shear stress for 24 h was associated with reduced internalisation of unmodified 80 nm spherical gold nanoparticles (AuNPs) (mean hydrodynamic size of 99 nm in culture medium) after exposure to flow conditions compared with cells that were cultured and exposed to static conditions. Under static conditions, targeting of 80 nm AuNPs conjugated with antibodies against the intracellular adhesion molecule 1 (ICAM-1) (mean hydrodynamic size of 109 nm in culture medium) markedly increased the internalisation of AuNPs in HUVECs that were activated with the tumour necrosis factor (TNF), a treatment that markedly increased the surface expression of ICAM-1. Shear stress-adapted and TNF-activated HUVECs, which were exposed to flow conditions, had higher association with anti-ICAM-1 AuNPs than cells that were not TNF-activated or exposed to particles under static conditions. Hence, shear stress adaptation reduces the uptake of unmodified AuNPs and increases the association between anti-ICAM-1 AuNPs and TNF-activated HUVECs.

Graphical abstract: The influence of flow, shear stress and adhesion molecule targeting on gold nanoparticle uptake in human endothelial cells

Back to tab navigation

Supplementary files

Article information


Submitted
06 Mar 2015
Accepted
21 May 2015
First published
25 May 2015

Nanoscale, 2015,7, 11409-11419
Article type
Paper

The influence of flow, shear stress and adhesion molecule targeting on gold nanoparticle uptake in human endothelial cells

H. Klingberg, S. Loft, L. B. Oddershede and P. Møller, Nanoscale, 2015, 7, 11409
DOI: 10.1039/C5NR01467K

Social activity

Search articles by author

Spotlight

Advertisements