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Issue 15, 2015
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Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2−x NPs

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Abstract

Cerium Oxide nanoparticles (CeO2−x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2−x NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2−x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2−x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO2−x NPs. The brush coating does not prevent CeO2−x NPs from displaying antioxidant properties.

Graphical abstract: Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2−x NPs

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Supplementary files

Article information


Submitted
06 Feb 2015
Accepted
10 Mar 2015
First published
12 Mar 2015

This article is Open Access

Nanoscale, 2015,7, 6588-6598
Article type
Paper
Author version available

Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2−x NPs

Y. Qiu, E. Rojas, R. A. Murray, J. Irigoyen, D. Gregurec, P. Castro-Hartmann, J. Fledderman, I. Estrela-Lopis, E. Donath and S. E. Moya, Nanoscale, 2015, 7, 6588
DOI: 10.1039/C5NR00884K

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  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
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    [Original citation] - Published by the PCCP Owner Societies.
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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


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