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Issue 8, 2015
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An ultra-sensitive impedimetric immunosensor for detection of the serum oncomarker CA-125 in ovarian cancer patients

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Abstract

Effective treatment of ovarian cancer depends upon the early detection of the malignancy. Here, we report on the development of a new nanostructured immunosensor for early detection of cancer antigen 125 (CA-125). A gold electrode was modified with mercaptopropionic acid (MPA), and then consecutively conjugated with silica coated gold nanoparticles (AuNP@SiO2), CdSe quantum dots (QDs) and anti-CA-125 monoclonal antibody (mAb). The engineered MPA|AuNP@SiO2|QD|mAb immunosensor was characterised using transmission electron microscopy (TEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Successive conjugation of AuNP@SiO2, CdSe QD and anti-CA-125 mAb onto the gold electrode resulted in sensitive detection of CA-125 with a limit of detection (LOD) of 0.0016 U mL−1 and a linear detection range (LDR) of 0–0.1 U mL−1. Based on the high sensitivity and specificity of the immunosensor, we propose this highly stable and reproducible biosensor for the early detection of CA-125.

Graphical abstract: An ultra-sensitive impedimetric immunosensor for detection of the serum oncomarker CA-125 in ovarian cancer patients

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Publication details

The article was received on 12 Nov 2014, accepted on 17 Jan 2015 and first published on 19 Jan 2015


Article type: Paper
DOI: 10.1039/C4NR06687A
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Citation: Nanoscale, 2015,7, 3768-3779

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    An ultra-sensitive impedimetric immunosensor for detection of the serum oncomarker CA-125 in ovarian cancer patients

    M. Johari-Ahar, M. R. Rashidi, J. Barar, M. Aghaie, D. Mohammadnejad, A. Ramazani, P. Karami, G. Coukos and Y. Omidi, Nanoscale, 2015, 7, 3768
    DOI: 10.1039/C4NR06687A

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