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Issue 8, 2015
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Application of an optimized electrochemical sensor for monitoring astaxanthin antioxidant properties against lipoperoxidation

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Abstract

An optimized electrochemical sensor was developed to assess the antioxidant capacity of carotenoids, accumulating during the life cycle of Haematococcus pluvialis cell cultures. The sensor was improved with a composite renewable surface made of immobilised phosphatidylcholine (PC) on magnetic nanobeads of iron oxide (Fe3O4) and PC/Fe3O4, and it was used to monitor the antioxidant properties of the ketocarotenoid astaxanthin against in situ generated phosphatidylcholine lipoperoxides. The surface configuration was able to mimic the natural position and orientation of astaxanthin in the cellular membrane, conferring to the whole experimental set-up good sensitivity for reactive oxygen species (limit of detection for peroxyl radicals 9.1 × 10−10 mol L−1) with a linear response ranging between 10−8 and 1.6 × 10−6 mol L−1. The sensor has been proved suitable for both batch and flow measurements. The accuracy of the flow measurements was unaffected by the magnetic field intensity. Electrochemical measurements confirmed that natural astaxanthin is a more effective antioxidant than synthetic astaxanthin, vitamin E and lutein and the protective effect of astaxanthin correlates with its concentration inside the cell. The newly developed sensor is hence useable for in-line monitoring of whole-cell based industrial bioprocesses for the production of astaxanthin.

Graphical abstract: Application of an optimized electrochemical sensor for monitoring astaxanthin antioxidant properties against lipoperoxidation

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Supplementary files

Article information


Submitted
23 Feb 2015
Accepted
09 Jun 2015
First published
10 Jun 2015

New J. Chem., 2015,39, 6428-6436
Article type
Paper
Author version available

Application of an optimized electrochemical sensor for monitoring astaxanthin antioxidant properties against lipoperoxidation

R. Penu, S. C. Litescu, S. A. V. Eremia, I. Vasilescu, G. Radu, M. T. Giardi, G. Pezzotti and G. Rea, New J. Chem., 2015, 39, 6428
DOI: 10.1039/C5NJ00457H

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